Nanoplate digital PCR assays for detection and quantification of Xylella fastidiosa

Xylella fastidiosa is a fastidious Gram-negative bacterium that associated with several important plant diseases, and regulated as quarantine pest in many countries where strategies are implemented to prevent its introduction spread. To enact efficient measures, effective early detection of the pathogen essential, especially because global trade goods increases risks alien pathogens. this study aimed adapt two qPCR-based diagnostic methods (SYBR Green Probe based qPCR), already use detect X. fastidiosa, for nanoplate digital PCR assay. Detection using assays (EvaGreen- Probe-based) was similar standard qPCR, giving 100% sensitivity, specificity, accuracy, while providing accurate absolute quantification when experimental samples had low concentrations host DNA. Using undiluted DNA added only TaqMan method maintained satisfactory performance quantification, therefore preferred. These results first step demonstrating usefulness nanoplate-based pathogens, which allows greater throughput than reducing time cost assays.